產(chǎn)品貨號(hào)

BP0101

產(chǎn)品規(guī)格

5/25/50/100mg

反應(yīng)種屬

Mouse

克隆號(hào)

10F.9G2

同種型

Rat IgG2b, κ

免疫原

Mouse CD274

實(shí)驗(yàn)應(yīng)用

in vivo PD-L1 blockade
Immunofluorescence
Immunohistochemistry (frozen)
Flow cytometry
Western blot

產(chǎn)品形式

PBS, pH 6.5,Contains no stabilizers or preservatives

純度

>95%, Determined by SDS-PAGE

聚合

<5%, Determined by SEC

無(wú)菌處理

0.2 µm filtration

純化方式

Protein G

RRID

AB_10949073

分子量

150 kDa

小鼠病原檢測(cè)

Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

保存條件

抗體原液保存在4°C，不能冷凍保存。

推薦同型對(duì)照

InVivoPlus rat IgG2b isotype control, anti-keyhole limpet hemocyanin（貨號(hào)BP0090）

推薦抗體稀釋液

InVivoPure pH 6.5 Dilution Buffer（貨號(hào)IP0065）

體內(nèi)PD-L1阻斷

(in vivo PD-L1 blockade)

1. Grasselly, C., et al. (2018). 'The Antitumor Activity of Combinations of Cytotoxic Chemotherapy and Immune Checkpoint Inhibitors Is Model-Dependent' Front Immunol 9: 2100.

2. Stathopoulou, C., et al. (2018). 'PD-1 Inhibitory Receptor Downregulates Asparaginyl Endopeptidase and Maintains Foxp3 Transcription Factor Stability in Induced Regulatory T Cells' Immunity 49(2): 247-263 e247.

3. Kim, J., et al. (2015). 'Memory programming in CD8(+) T-cell differentiation is intrinsic and is not determined by CD4 help' Nat Commun 6: 7994.

體內(nèi)PD-L1阻斷，流式細(xì)胞術(shù)

(in vivo PD-L1 blockade, Flow Cytometry)

1. Aloulou, M., et al. (2016). 'Follicular regulatory T cells can be specific for the immunizing antigen and derive from naive T cells' Nat Commun 7: 10579.

2. Ngiow, S. F., et al. (2015). 'A Threshold Level of Intratumor CD8+ T-cell PD1 Expression Dictates Therapeutic Response to Anti-PD1' Cancer Res 75(18): 3800-3811.

3. Rutigliano, J. A., et al. (2014). 'Highly pathological influenza A virus infection is associated with augmented expression of PD-1 by functionally compromised virus-specific CD8+ T cells' J Virol 88(3): 1636-1651.

體內(nèi)PD-L1阻斷，免疫熒光

(in vivo PD-L1 blockade, Immunofluorescence)

1.Willimsky, G., et al. (2013). 'Virus-induced hepatocellular carcinomas cause antigen-specific local tolerance' J Clin Invest 123(3): 1032-1043.

免疫組織化學(xué)（冷凍切片），免疫熒光

(Immunohistochemistry (frozen), Immunofluorescence)

1.Riella, L. V., et al. (2011). 'Essential role of PDL1 expression on nonhematopoietic donor cells in acquired tolerance to vascularized cardiac allografts' Am J Transplant 11(4): 832-840.